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High-level expression of a biologically active staphylokinase in Pichia pastoris.
Faraji H, Ramezani M, Sadeghnia HR, Abnous K, Soltani F, Mashkani B. Faraji H, et al. Prep Biochem Biotechnol. 2017 Apr 21;47(4):379-387. doi: 10.1080/10826068.2016.1252924. Epub 2016 Nov 4. Prep Biochem Biotechnol. 2017. PMID: 27813714
The expression of recombinant SAK (rSAK) reached to its maximum (310 mg/L of the culture medium) after 48-hr stimulation with 3% methanol and remained steady until day 5. ...
The expression of recombinant SAK (rSAK) reached to its maximum (310 mg/L of the culture medium) after 48-hr stimulation with 3% meth …
Comparison of expression optimization of new derivative of staphylokinase (SAK-2RGD-TTI) with the rSAK.
Faraji H, Ramezani M, Mashkani B, Sadeghnia HR, Benhangi HM, Hosseini Teshnizi S, Soltani F. Faraji H, et al. Biotechnol Prog. 2019 Jul;35(4):e2819. doi: 10.1002/btpr.2819. Epub 2019 Apr 25. Biotechnol Prog. 2019. PMID: 30972956
Expression level of the designed construct reached up to 175 mg/L of the culture medium after 72-hr stimulation with 2.5% methanol and remained steady for 3-4 days. ...
Expression level of the designed construct reached up to 175 mg/L of the culture medium after 72-hr stimulation with 2.5% methanol an …
Cytotoxic effects of auraptene against a human malignant glioblastoma cell line.
Afshari AR, Karimi Roshan M, Soukhtanloo M, Ghorbani A, Rahmani F, Jalili-Nik M, Vahedi MM, Hoseini A, Sadeghnia HR, Mollazadeh H, Mousavi SH. Afshari AR, et al. Avicenna J Phytomed. 2019 Jul-Aug;9(4):334-346. Avicenna J Phytomed. 2019. PMID: 31309072 Free PMC article.
MATERIALS AND METHODS: The cellular toxicity was measured by the MTT assay following 24 and 48-hr treatment with different concentrations of auraptene (0-400mug/ml). ...RESULTS: The results revealed that auraptene reduced the viability of U87 cells concentration- and time- …
MATERIALS AND METHODS: The cellular toxicity was measured by the MTT assay following 24 and 48-hr treatment with different concentrat …
Effect of rutin on oxidative DNA damage in PC12 neurons cultured in nutrients deprivation condition.
Nassiri-Asl M, Ghorbani A, Salehisar S, Asadpour E, Sadeghnia HR. Nassiri-Asl M, et al. Iran J Basic Med Sci. 2020 Mar;23(3):390-395. doi: 10.22038/IJBMS.2020.31832.7657. Iran J Basic Med Sci. 2020. PMID: 32440327 Free PMC article.
This work aimed to examine whether rutin can protect neurons against oxidative DNA damage caused by serum/glucose deprivation (SGD) as an in vitro model of neurodegeneration and ischemia. MATERIALS AND METHODS: The PC12 cells were cultured for 2 hr in normal culture medium …
This work aimed to examine whether rutin can protect neurons against oxidative DNA damage caused by serum/glucose deprivation (SGD) as an in …
Berberine protects against glutamate-induced oxidative stress and apoptosis in PC12 and N2a cells.
Sadeghnia HR, Kolangikhah M, Asadpour E, Forouzanfar F, Hosseinzadeh H. Sadeghnia HR, et al. Iran J Basic Med Sci. 2017 May;20(5):594-603. doi: 10.22038/IJBMS.2017.8847. Iran J Basic Med Sci. 2017. PMID: 28656094 Free PMC article.
MATERIALS AND METHODS: The cultured PC12 and N2a cells were pretreated (2 hr) with varying concentrations of berberine (50-1000 M), followed by exposure to glutamate (10 mM) for 24 hr. ...
MATERIALS AND METHODS: The cultured PC12 and N2a cells were pretreated (2 hr) with varying concentrations of berberine (50-1000 M), f …
Cuscuta campestris induces apoptosis by increasing reactive oxygen species generation in human leukemic cells.
Moradzadeh M, Hosseini A, Rakhshandeh H, Aghaei A, Sadeghnia HR. Moradzadeh M, et al. Avicenna J Phytomed. 2018 May-Jun;8(3):237-245. Avicenna J Phytomed. 2018. PMID: 29881709 Free PMC article.
In this study, we examined the level of cellular oxidants, cytotoxicity, apoptosis and differentiation induced by hydroalcoholic extract of C. campestris (CCE) (12.5-200 g/ml), as well as arsenic trioxide (As(2)O(3), 50 M), in human leukemic (HL60 and NB4) and normal polymorph nu …
In this study, we examined the level of cellular oxidants, cytotoxicity, apoptosis and differentiation induced by hydroalcoholic extract of …
Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage.
Havakhah S, Sadeghnia HR, Hajzadeh MA, Roshan NM, Shafiee S, Hosseinzadeh H, Mohareri N, Rad AK. Havakhah S, et al. Iran J Basic Med Sci. 2014 Dec;17(12):986-92. Iran J Basic Med Sci. 2014. PMID: 25859302 Free PMC article.
NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-150 and 300) or at the beginning of reperfusion phase (T-150 and 300), via jugular catheter intravenously. ...
NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-1 …